منابع مشابه
HPRT targeting: "Ets" a powerful tool for investigating endothelial-cell specific gene expression.
Elucidating the role of genes that are important for the development of the vascular endothelium and their time course of expression throughout ontogeny is an important and exciting area of ongoing investigation. In addition to advances that can be made to better understand the vasculature from a developmental perspective, there are obvious clinical implications from determining how gene expres...
متن کاملTargeting the Hprt locus in mice reveals differential regulation of Tie2 gene expression in the endothelium.
To study the in vivo expression of the murine Tie2 gene, we have targeted the hypoxanthine phosphoribosyltransferase (Hprt) gene locus to generate two single-copy transgenic mice: T1, containing the 2,100-bp Tie2 promoter upstream from the beta-galactosidase (LacZ) gene, and T5, which also included an enhancing element originating from the first intron of the Tie2 gene. Comparing T1 and T5 embr...
متن کاملEnriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene
The CRISPR-Cas9 system uses guide RNAs to direct the Cas9 endonuclease to cleave target sequences. It can, in theory, target essentially any sequence in a genome, but the efficiency of the predicted guide RNAs varies dramatically. If no targeted cells are obtained, it is also difficult to know why the experiment fails. We have developed a transient transfection based method to enrich successful...
متن کاملHprt mutant frequency and molecular analysis of Hprt mutations in Fischer 344 rats treated with thiotepa.
Thiotepa is a bifunctional alkylating anticancer drug that is a rodent carcinogen and a suspected human carcinogen. In order to determine the sensitivity of mutant induction in the Hprt lymphocyte assay for detecting tumorigenic doses of thiotepa, Fischer 344 rats were treated for 4 weeks with thiotepa using a procedure adapted from a carcinogenesis protocol. At various times after beginning th...
متن کاملHPRT: gene structure, expression, and mutation.
INTRODUCTION AND PERSPECTIVES ....................................................... 127 NORMAL ENZYME FUNCTION AND CHARACTERISTICS ............................. 129 Enzymology ........................................................................................ 129 Tissue Distribution ................................................................................ 130 HPRT Gene Structure ....
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ژورنال
عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology
سال: 2003
ISSN: 1079-5642,1524-4636
DOI: 10.1161/01.atv.0000096608.44578.d9